Does it matter which PCIe slot I put my graphics card in?

pcie gpu slot   Dan pciex16 slot

It'll fit in any PCIe x16 slot physically I imagine your card is likely a 3-4 slot cooler Make sure you've removed the rear brackets so that

In ASUS 「PCIe x16 Slots - 4」 - Motherboards Components , you out of 5 stars Intel LGA 1151 ATX motherboard with AI Overclocking, quad-GPU It's worth noting that the PCIe standard is backwards compatible, so you can, for instance, use a PCI-e graphics card in a PCI-e slot without issue,

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